Luciferase Immunoprecipitations System

An introduction to the work performed developing the Lassa LIPS assay.

David Simons
04-06-2021

To support our ongoing work on understanding the epidemiology and transmission dynamics in multi-species systems in endemic regions we have begun developing a cross-species assay. We adopted an approach that has been found to be successful for Peste des petits ruminants a virus that affects goats, sheep and camels and is targeted for eradication. These assays are built on the Luciferase Immunoprecipitation System (Berguido et al. 2016) which detect the presence of antibodies against the presented antigen.

Figure 1 shows a schematic approach to the LIPS assay

LIPS assay CC-BY-NC B. Drame

Figure 1: LIPS assay CC-BY-NC B. Drame

Identification of potential antigenic epitopes

A bioinformatic approach was implemented to identify potential antigen epitopes from previously published sequences on NCBI. This work was written up to be presented at the cancelled ICREID 2020 conference. Below is the poster we produced to describe the workflow for the production of epitopes.

Poster produced for ICREID conference

Figure 2: Poster produced for ICREID conference

Berguido, Francisco J., Sanne Charles Bodjo, Angelika Loitsch, and Adama Diallo. 2016. “Specific Detection of Peste Des Petits Ruminants Virus Antibodies in Sheep and Goat Sera by the Luciferase Immunoprecipitation System.” Journal of Virological Methods 227 (January): 40–46. https://doi.org/10.1016/j.jviromet.2015.10.008.

References

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Citation

For attribution, please cite this work as

Simons (2021, April 6). David Simons: Luciferase Immunoprecipitations System. Retrieved from wwww.dsimons.org/posts/2021-04-06-luciferase-immunoprecipitations-system/

BibTeX citation

@misc{simons2021luciferase,
  author = {Simons, David},
  title = {David Simons: Luciferase Immunoprecipitations System},
  url = {wwww.dsimons.org/posts/2021-04-06-luciferase-immunoprecipitations-system/},
  year = {2021}
}